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With its core technology in place, PacBio was ready to go commercial. In 2011, the company released the RS sequencing machine, and has since created multiple new machines containing chips with increased numbers of sequencing wells. PacBio calls the technique single molecule real time (SMRT) sequencing, though it’s colloquially referred to simply as PacBio sequencing. Rather than producing short overlapping reads like Illumina, PacBio generates very long reads; at first these were a few thousand bases, but today they can be well over 10,000.

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This metaphor has been present in essentially every Smalltalk with a full IDE (and yes: there are Smalltalk systems without a traditional IDE, like GemStone or GNU Smalltalk). It has collected both praise and criticism. What’s striking is that it has not been displaced.

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